Christa N. Hestekin
Assistant Professor
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Dr. Hestekin's research interests are in the separation of biomolecules, specifically DNA, using microchannel electrophoresis for applications in medicine, agriculture, environmental sciences, and biosecurity. Electrophoresis is the use of an electric field to separate charged molecules based on a difference in electrophoretic mobility, which can result from differences in charge and/or shape or size. Dr. Hestekin is a member of the American Institute of Chemical Engineers and the American Electrophoresis Society.
Education
B.S. Chemical Engineering, University of Kentucky, 2000
Selected Publications
C.N. Hestekin, D.Y. Kim, J.P. Jakupciak, C. O'Connell, and A.E. Barron, "Optimization of Polymer Matrix and Sample Preparation for Development of a Rapid, Sensitive Mutation Detection of the p53 Gene using Microchip Electrophoresis-SSCP/HA," to be submitted
C.N. Hestekin, L. Senderowicz, J.P. Jakupciak, C. O'Connell, A. Rademaker, and A.E. Barron, "Blinded study determination of high sensitivity and specificity of microchip electrophoresis – tandem SSCP/HA to detect mutations in the p53 gene," to be submitted
I.V. Kourkine, C.N. Hestekin, B.A. Buchholz, and A.E. Barron, "High-throughput, high sensitivity genetic mutation detection by tandem single strand conformation polymorphism (SSCP) – heteroduplex analysis (HA) capillary array electrophoresis (CAE)," Anal.Chem., 74 2565-2572 (2002)
I.V. Kourkine, C.N. Hestekin, S. Magnusdottir, and A.E. Barron, "Optimized Sample Preparation for Tandem Capillary Electrophoresis Single-Strand Conformation Polymorphism/Heteroduplex Analysis (CE-SSCP/HA)," BioTechniques, 33 318-325 (2002)
I.V. Kourkine, C.N. Hestekin, and A.E. Barron, "Technical Challenges in Applying Capillary Electrophoresis (CE)-Single Strand Conformation Polymorphism (SSCP) for Routine Genetic Analysis," Electrophoresis, 23 1375-1385 (2002)
J. A. Hestekin, C. N. Smothers (Hestekin), and D. Bhattacharyya, "Nanofiltration of Charged Organic Molecules in Aqueous and Non-Aqueous Solvents,"in Membrane Technology in the Chemical Industry, (ed. by Nunes and Peinemann), Wiley-VCH, Invited Chapter, p 173-190 (2001)
M. E. Williams, J. A. Hestekin, C. N. Smothers (Hestekin), and D. Bhattacharyya, "Separation of Organic Pollutants by Reverse Osmosis and Nanofiltration Membranes: Mathematical Models and Experimental Verification", Ind. Eng. Chem. Res.,38 3683-3695 (1999).
